Facilities
Highlights
Asylum MFP-3D-BIO AFM on Olympus IX81 microscope
Description:
The Asylum MFP-3D AFM is integrated with Olympus IX81 inverted microscope. It provides the capability of simultaneous atomic force microscopy and fluorescence microscopy imaging. Extended AFM scanner provides up to 40um Z range for cell imaging. AFM tip is monitored by superluminescent diode with an 860nm wavelength to achieve a flat baseline in force curves and images, and enable use of red fluorophores such as (TXRED, Cy5). Closed fluid cell and BioHeater are included to conduct dynamic experiments and maintain living cells under physiological temperatures. Fluorescence excitation is provided by X-Cite(120) white light source with three selectable Olympus fluorescence filters for DAPI(358nm), GFP(488nm) and TXRED (595nm) dyes. IX81 inverted microscope currently has 10X, 20X and 40X objective lenses. Fluorescence image is captured by Hamamatsu ORCA-AG camera with high sensitivity in VIS-NIR region.
For more information contact Larry Millet (millet@ilfe.illinois.edu)
Email to all Asylum AFM users (MNTL-Asylum-Users@ad.uiuc.edu)
AFM Training Video by Asylum Research Scientist Dr. Nicholas Geisse
Introduction to AFM (~2hr)
Training Video 1-- AC mode imaging in air (~2hr)
Training Video 2 -- Calibration of cantilever and force curve (~30min)
Training Video 3 -- Contact mode imaging in air (~20min)
Training Video 4 -- Contact mode and AC mode imaging in liquid (~40min)
Training Video 5 -- BioHeater (~35min)
Optical Microscope Training Video (~10min)
Humidity Control Flow Cell
Introduction for humiditiy control flow cell
Putting the cell onto the head
Last Updated: Jan 2009
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AFM image of collagen fiber with repeated 66nm binding | |
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AFM image of cell in PBS solution |